NAD+ 500mg research compound lyophilised powder

Metabolic Research

NAD+

Name: NAD+ 500mg
Brand: UK Peptide Lab
SKU: UKPL-687
Price: 49.99 GBP
Availability: InStock

500mg

Cellular redox coenzyme. Mitochondrial and sirtuin pathway research compound.

£49.99In Stock

Third-party tested · Batch UKPL-6235 · View certificate

Size

500mg£49.99£0.100/mg 1000mg£84.99£0.085/mg

Quantity

Same-day dispatch before 3pm, Mon to Fri
Add £19.01 more for free UK delivery
Cold-chain shipping. Always. Dry-cold pack, Royal Mail Tracked 24
Secure checkout
Lost, damaged or wrong? Replaced free if you tell us within 48 hours.
Research use only. For in-vitro laboratory research. Not for use in humans or animals, or in food, drugs or diagnostics. Not evaluated by the MHRA or FDA. The buyer is responsible for handling and regulatory compliance.

Bulk Pricing

Counted across your basket, any mix of products.

Vials in basket	Discount	Per vial
1	None	£49.99
3-4	5%	£47.49save £2.50
5-9	8%	£45.99save £4.00
10-50	12%	£43.99save £6.00

Don't Forget

Bacteriostatic Water 10ml

Required to reconstitute lyophilised peptides.

£4.99

What is NAD+?

NAD+ (nicotinamide adenine dinucleotide, also written NAD plus) is the oxidised form of the cell's main redox cofactor. It is a dinucleotide, not a peptide. We supply it as lyophilised powder in 500mg and 1000mg vials.

Full description

The formula is C₂₁H₂₇N₇O₁₄P₂, the molecular weight 663.43 g/mol and the CAS number 53-84-9. Nicotinamide mononucleotide is joined to adenosine monophosphate through a pyrophosphate bridge. Redox chemistry happens on the nicotinamide ring, which accepts a hydride to give NADH.

Signalling enzymes such as sirtuins and PARPs consume NAD+ as a substrate (Liu et al., Cell Metab, 2018). Cells rebuild it through salvage pathways from nicotinamide, nicotinic acid or nicotinamide riboside. NAMPT catalyses the first step from nicotinamide (Grozio et al., J Biol Chem, 2013).

NAD+ Research Applications

Signalling: NAD+ activates sirtuins and supports mitochondrial function, including through the mitochondrial unfolded protein response (Cantó, Menzies and Auwerx, Cell Metab, 2015).
Turnover: isotope tracing in cell lines showed NAD made from nicotinamide and consumed largely by PARPs and sirtuins (Liu et al., 2018).
CD38: Camacho-Pereira and colleagues (Cell Metab, 2016) identified CD38 as the main enzyme degrading the precursor NMN in vivo.

Study details

Typical in-vitro uses are dehydrogenase assays read at 340 nm, sirtuin, PARP and CD38 activity assays, and NAD+/NADH ratio measurements in bioenergetics work.

NAD+ vs NMN vs NR (Nicotinamide Riboside)

NAD+ is the finished dinucleotide. NMN (nicotinamide mononucleotide) and NR (nicotinamide riboside) are precursors upstream of it in the salvage pathways. At the cell surface, CD38 converts NMN to nicotinamide and CD73 converts it to NR. In human cells, CD73 enabled extracellular NMN to feed NAD+ synthesis inside the cell, and CD38 impaired it (Grozio et al., 2013). For cell-free and enzyme work, NAD+ is the cofactor added directly.

Research Overview

NAD+ (nicotinamide adenine dinucleotide, oxidised form, often written NAD plus where the charge symbol cannot be rendered) has the molecular formula C₂₁H₂₇N₇O₁₄P₂, a molecular weight of 663.43 g/mol, and CAS number 53-84-9. Structurally it is nicotinamide mononucleotide joined to adenosine monophosphate through a pyrophosphate bridge, and its redox chemistry is confined to the nicotinamide ring: reduction is a stereospecific two-electron hydride transfer to C4 giving NADH, with a standard reduction potential near -0.32 V at pH 7. That single couple carries the bulk of catabolic electron flow into complex I of the respiratory chain, which is why bioenergetics research reports the free NAD+/NADH ratio rather than absolute NAD+ concentration. The second half of nicotinamide adenine dinucleotide biology is non-redox and consumptive. Sirtuins, poly(ADP-ribose) polymerases and the CD38/CD157 ectoenzymes cleave the glycosidic bond between the nicotinamide and the ADP-ribosyl moiety, releasing nicotinamide stoichiometrically, so NAD+ is destroyed rather than recycled and must be resynthesised through the NAMPT-limited salvage pathway (Cantó, Menzies and Auwerx, Cell Metabolism, 2015). Camacho-Pereira and colleagues (Cell Metabolism, 2016) reported that CD38 expression and NADase activity rise with age in mice and drive tissue NAD+ decline alongside SIRT3-dependent mitochondrial dysfunction, while Liu and colleagues (Cell Metabolism, 2018) applied stable-isotope tracing to quantify NAD synthesis and breakdown fluxes directly and map tissue-specific turnover rather than inferring it from pool sizes. One mechanistic point matters to anyone designing experiments: intact NAD+ is not efficiently transported across the mammalian plasma membrane. Extracellular NAD+ is processed by surface ectoenzymes instead, with CD73 shown to generate nicotinamide riboside that is subsequently taken up (Grozio et al., Journal of Biological Chemistry, 2013). Laboratories buying NAD+ in the UK typically use it as a defined cofactor in oxidoreductase assays and in sirtuin, PARP and mitochondrial bioenergetics work.

Product Specifications

NAD+ · Technical Data

Molecular Formula: C₂₁H₂₇N₇O₁₄P₂
Molecular Weight: 663.43 g/mol
CAS Number: 53-84-9
Content: 609.54 mg verified (121.9% of claim)
Batch: UKPL-6235
Form: White lyophilised powder
Content assay: 609.54 mg verified (500 mg label claim)

Certificate of Analysis

Third-party tested

Batch: UKPL-6235
Purity: 609.54 mg content (121.9% of claim)
Method: Content assay
Lab: Janoshik Analytical
Tested: Sep 2026

View Lab Certificate Verify batch UKPL-6235 Janoshik Analytical report #229865 Verify this test on janoshik.com

Janoshik Analytical report #229865 for NAD+, batch UKPL-6235

Original Janoshik report. Tap to enlarge.

Need the certificate for an earlier batch? Request an older CoA

Storage and Reconstitution

Store NAD+ powder at -20°C, away from light and moisture, for up to 24 months. After reconstitution, keep the solution at 2-8°C and use it within 4 weeks. For longer storage, freeze single-use aliquots at -20°C and avoid repeated freeze-thaw cycles.

Vials are sold on their own, without bacteriostatic water for mixing or other supplies.

How to reconstitute and handle

Let the vial and the bacteriostatic water reach room temperature and swab both stoppers with alcohol. Add the water slowly down the inside wall of the vial and swirl gently until dissolved. Do not shake. Avoid alkaline buffers: NAD+ is fairly stable in acid but hydrolyses in base, at both the nicotinamide-ribose bond and the pyrophosphate link.

NAD+ is comparatively stable in acidic solution and labile in alkaline solution, where both the nicotinamide-ribose glycosidic bond and the pyrophosphate linkage are susceptible to hydrolysis. NADH shows the opposite pH profile, so keep reconstituted NAD+ cold and avoid basic buffers.
Oxidised NAD+ absorbs at 260 nm, and reduction to NADH adds the characteristic 340 nm band that is the standard spectrophotometric check on redox state and integrity. Prepare single-use aliquots and avoid repeated freeze-thaw cycles.

For the full method, see the peptide reconstitution guide and the reconstitution calculator.

Research References

Published research on NAD+ is indexed on PubMed. See our full UK buyer's guide for NAD+ and UK buyer's guide for NAD+ 500mg.

NAD+ Price (UK)

NAD+ is for sale in 2 vial sizes, priced from £49.99 for 500mg to £84.99 for 1000mg. Every size ships from our UK facility, each with its own batch certificate. UK delivery is £4.50 by Royal Mail Tracked 24, free over £69.

NAD+ · Price per vial

Vial	Price	Cost per mg	Availability
NAD+ 500mgThis page	£49.99	£0.100	In stock
NAD+ 1000mg	£84.99	£0.085Best value	In stock

Prices are in GBP per vial and match the basket. See the FAQ for delivery and payment options, and quality & testing for how each batch is checked.

Frequently Asked Questions

What is NAD+ (nicotinamide adenine dinucleotide)?

NAD+ is the oxidised form of nicotinamide adenine dinucleotide, a dinucleotide cofactor with the molecular formula C₂₁H₂₇N₇O₁₄P₂, a molecular weight of 663.43 g/mol and CAS number 53-84-9. It consists of nicotinamide mononucleotide linked to adenosine monophosphate through a pyrophosphate bridge. It is supplied as lyophilised powder for research use only.

Is NAD+ the same as NAD plus?

Yes. NAD plus is simply how the name is written when the superscript charge symbol cannot be rendered, for example in plain-text search boxes and product listings. Both refer to the oxidised form of nicotinamide adenine dinucleotide. The reduced form is written NADH.

What is the difference between NAD+ and NADH?

NAD+ and NADH are the oxidised and reduced members of the same redox couple. Reduction is a stereospecific two-electron hydride transfer to C4 of the nicotinamide ring, and the couple has a standard reduction potential near -0.32 V at pH 7. Only NADH carries the 340 nm absorbance band, which is why that wavelength is used to follow dehydrogenase reactions spectrophotometrically.

What is the difference between NAD+, NMN and NR?

NMN (nicotinamide mononucleotide) and NR (nicotinamide riboside) are biosynthetic precursors that sit upstream of NAD+ in the salvage pathway, whereas NAD+ is the finished cofactor. Because intact NAD+ is not efficiently taken up by cells, most in-vivo literature on raising tissue NAD+ works with precursors rather than the dinucleotide itself (Rajman, Chwalek and Sinclair, Cell Metabolism, 2018). All three are research compounds only.

Why does NAD+ decline with age in research models?

The prevailing explanation is increased consumption rather than reduced synthesis. Camacho-Pereira and colleagues (Cell Metabolism, 2016) showed that expression and NADase activity of the ectoenzyme CD38 rise with age in mice, degrading NAD+ and producing SIRT3-dependent mitochondrial dysfunction. Isotope-tracing work by Liu and colleagues (Cell Metabolism, 2018) has since quantified synthesis and breakdown fluxes tissue by tissue.

Can cells take up NAD+ directly?

Not efficiently. Intact NAD+ does not readily cross the mammalian plasma membrane; extracellular NAD+ is instead hydrolysed by cell-surface ectoenzymes, and CD73 has been shown to generate nicotinamide riboside that is then transported into the cell and reassembled (Grozio et al., Journal of Biological Chemistry, 2013). This is a central consideration when designing in-vitro NAD+ experiments.

What is in a NAD+ vial?

Each vial contains NAD+ as lyophilised powder, freeze-dried for stability. NAD+ has a molecular weight of 663.43 g/mol, molecular formula C₂₁H₂₇N₇O₁₄P₂, and CAS number 53-84-9. This compound is supplied for research use only and is not a medicine.

Where can I buy NAD+ in the UK?

You can buy NAD+ online from UK Peptide Lab, in 500mg and 1000mg vials from UK stock. Order before 3pm (UK time) on a working day for same-day dispatch. UK delivery is free over £69.

Is your NAD plus 500mg UK third-party tested?

Yes. The 500mg batch has a third-party certificate, published in the certificate section. It is a content assay: it reports the NAD+ found in the vial against the label claim. The 1000mg vial has its own certificate, which reports HPLC purity.

What is NAD+ used for in research?

In-vitro enzymology and bioenergetics: dehydrogenase assays, sirtuin, PARP and CD38 activity, and NAD+/NADH ratio work.

Research Use Disclaimer